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Multiple culture instances are often required and timing is critical Laboratories outside of the united states may participate in this proficiency test and are not subject to the us laboratory approval process. Official cem testing generally involves multiple sets of samples taken on multiple days
Exact sampling schedules need to be confirmed with appropriate regulatory agencies in advance of testing All laboratory personnel who perform cem culture testing are required to take and pass this proficiency test when offered Contaminated culture results will require.
How is contagious equine metritis diagnosed
Bacterial culture and the complement fixation (cf) test are the official tests for cem, with culture being the gold standard Identification of carriers relies on isolation of the bacteria from urogenital swabs. Prophylactic, annual testing of stallions prior to the breeding season as a means of reducing the risk of spread has been proposed and is recommended by usda for active breeding stallions. Ovariectomized mares (mares that have had their ovaries removed) are used for test breeding so that they can be synchronized easily and no pregnancy can result.
This document outlines procedures for testing imported horses for permanent entry from regions which the animal and plant health inspection service (aphis) considers affected with cem as well as the approval criteria for cem quarantine facilities. What tests are available to diagnose cem There are two official validated diagnostic tests for cem Bacterial culture and the serologic complement fixation (cf) test
Bacterial culture is the gold standard test to detect the organism from swabs collected from the genitalia of stallions and mares.
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