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The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches. Blast can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.

The basic local alignment search tool (blast) finds regions of similarity between sequences The basic local alignment search tool (blast) finds regions of local similarity between protein or nucleotide sequences The program compares nucleotide or protein sequences and calculates the statistical significance of matches.

Standard nucleotide blast blastn programs search nucleotide databases using a nucleotide query

Blast is also often used as part of other algorithms that require approximate sequence matching Blast is available on the web on the ncbi website Different types of blasts are available according to the query sequences and the target databases. Then use the blast button at the bottom of the page to align your sequences

To get the cds annotation in the output, use only the ncbi accession or gi number for either the query or subject. Blastp smart blast searches a protein query against the landmark database enter protein query sequence

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